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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in lots of tumors, which leads to chromosome instability, and so may perhaps add to tumorigenesis. This protein was found to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital part in marketing mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been explained. Two further splice variants were claimed but thorough nucleotide sequence details is not still obtainable.
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Blocking peptides are peptides that bind specially towards the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged with the antibody.
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This tutorial outlines additional attainable optimization of mobile and immunoassay parameters to ensure the best possible benefits are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide no more bind to your epitope to the goal protein. By comparing the staining from the blocked antibody vs . the antibody by yourself, one can see which staining is particular.
Concentrate on Facts Cyclin E belongs for the extremely conserved cyclin family, whose customers are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins show distinct expression and degradation designs which add for the temporal coordination of each and every mitotic function. Cyclin E sorts a complex with and features as a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates at the G1-S stage boundary and is particularly degraded as cells progress by means of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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